TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Koch, Marcus
A1  - Apushkinskaya, Nina
A1  - Zolotukhina, Ekaterina V.
A1  - Silina, Yuliya E.
T1  - Towards hybrid one-pot/one-electrode Pd-NPs-based nanoreactors for modular biocatalysis
JF  - Biochemical Engineering Journal
N2  - Here, fundamental aspects affecting template-assisted engineering of oxidase-associated peroxide oxidation co-catalysis of the modeled microanalytical system based on the hybrid palladium nanoparticles (Pd-NPs) with tailored functional properties were studied. By an accurate tuning and validation of the experimental setup, a modular Pd-NPs-doped one-pot/one-electrode amperometric nanobiosensor for advanced multiplex analyte detection was constructed. The specific operational conditions (electrochemical read-out mode, pH, regeneration procedure) of the modular one-pot/one-electrode nanobiosensor allowed a reliable sensing of L-lactate (with linear dynamic range, LDR=500 µM – 2 mM, R2 =0.977), D-glucose (with LDR=200 µM – 50 mM, R2 =0.987), hydrogen peroxide (with LDR=20 µM – 100 mM, R2 =0.998) and glutaraldehyde (with LDR=1 – 100 mM, R2 =0.971). In addition, mechanistic aspects influencing the performance of Pd-NPs-doped one-pot/one-electrode for multiplex analyte sensing were studied in detail. The designed one-pot/one-electrode amperometric nanobiosensor showed a thin layer electrochemical behavior that greatly enhanced electron transfer between the functional hybrid layer and the electrode. Finally, a specific regeneration procedure of the hybrid one-pot/one-electrode and algorithm towards its usage for modular biocatalysis were developed. The reported strategy can readily be considered as a guideline towards the fabrication of commercialized nanobiosensors with tailored properties for advanced modular biocatalysis.
KW  - One-pot/one-electrode nanobiosensor
KW  - Multiplexed analysis
KW  - Small molecular weight bioanalytes
KW  - Read-out mode
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-1077
U6  - https://doi.org/10.1016/j.bej.2021.108132
DO  - https://doi.org/10.1016/j.bej.2021.108132
VL  - 175
SP  - 108132
ER  -