TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Chen, Yaping
A1  - Yoh, Hao Zhe
A1  - Shokouhi, Ali-Reza
A1  - Murayama, Takahide
A1  - Suu, Koukou
A1  - Morikawa, Yasuhiro
A1  - Voelcker, Nicolas H.
A1  - Elnathan, Roey
T1  - Role of actin cytoskeleton in cargo delivery mediated by vertically aligned silicon nanotubes
JF  - Journal of Nanobiotechnology
N2  - Nanofabrication technologies have been recently applied to the development of engineered nano–bio interfaces for manipulating complex cellular processes. In particular, vertically configurated nanostructures such as nanoneedles (NNs) have been adopted for a variety of biological applications such as mechanotransduction, biosensing, and intracellular delivery. Despite their success in delivering a diverse range of biomolecules into cells, the mechanisms for NN-mediated cargo transport remain to be elucidated. Recent studies have suggested that cytoskeletal elements are involved in generating a tight and functional cell–NN interface that can influence cargo delivery. In this study, by inhibiting actin dynamics using two drugs—cytochalasin D (Cyto D) and jasplakinolide (Jas), we demonstrate that the actin cytoskeleton plays an important role in mRNA delivery mediated by silicon nanotubes (SiNTs). Specifically, actin inhibition 12 h before SiNT-cellular interfacing (pre-interface treatment) significantly dampens mRNA delivery (with efficiencies dropping to 17.2% for Cyto D and 33.1% for Jas) into mouse fibroblast GPE86 cells, compared to that of untreated controls (86.9%). However, actin inhibition initiated 2 h after the establishment of GPE86 cell–SiNT interface (post-interface treatment), has negligible impact on mRNA transfection, maintaining > 80% efficiency for both Cyto D and Jas treatment groups. The results contribute to understanding potential mechanisms involved in NN-mediated intracellular delivery, providing insights into strategic design of cell–nano interfacing under temporal control for improved effectiveness.
KW  - silicone nanotubes
KW  - actin inhibition
KW  - cytoskeleton
KW  - intracellular delivery
KW  - nanoinjection
Y1  - 2022
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-1845
U6  - https://doi.org/10.1186/s12951-022-01618-z
DO  - https://doi.org/10.1186/s12951-022-01618-z
VL  - 20
IS  - 1
SP  - 406
ER  -