TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Kocer, Gülistan
A1  - Albino, Ines M. C.
A1  - Verheijden, Mark L.
A1  - Jonkheijm, Pascal
T1  - Endothelial cell spreading on lipid bilayers with combined integrin and cadherin binding ligands
JF  - Bioorganic & Medicinal Chemistry
N2  - Endothelial cells play a central role in the vascular system, where their function is tightly regulated by both cell-extracellular matrix (e.g., via integrins) and cell–cell interactions (e.g., via cadherins). In this study, we incorporated cholesterol-modified integrin and N-cadherin peptide binding ligands in fluid supported lipid bilayers. Human umbilical vein endothelial cell adhesion, spreading and vinculin localization in these cells were dependent on ligand density. One composition led to observe a higher extent of cell spreading, where cells exhibited extensive lamellipodia formation and a qualitatively more distinct N-cadherin localization at the cell periphery, which is indicative of N-cadherin clustering and a mimic of cell–cell contact formation. The results can be used to reconstitute the endothelial-pericyte interface on biomedical devices and materials.
KW  - cell-surface interactions
KW  - surface modification
KW  - supported lipid bilayers
KW  - cell-instructive
KW  - peptide ligands
Y1  - 2022
U6  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-1896
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-1896
VL  - 68
SP  - 116850
ER  -