@phdthesis{Joseph2021,
  author    = {Desna Joseph},
  title     = {Light responsive cell-cell like biointerface using cadherin peptidomimetics},
  address   = {Saarbr{\"u}cken},
  doi       = {10.22028/D291-35156},
  url       = {https://nbn-resolving.org/urn:nbn:de:bsz:291:415-2094},
  pages     = {XX, 130 S.},
  year      = {2021},
  abstract  = {Interaction between the cells plays a vital role in tissue formation and transmembrane signalling. The family of Cadherin proteins is among the important classes of adhesion molecules engaged in cell-cell interactions. Whose members mediate homophilic Ca2+ dependent cell-cell adhesion, and they are dynamic and spatiotemporally tightly regulated in a wide variety of tissues. Dynamic control of cadherin mediated interactions is a key to understand and modulate various cellular events for biomedical applications. This thesis presents a new strategy to spatiotemporally control cadherin mediated cell-cell interactions at biomimetic interfaces. For this purpose photoactivatable peptidomimetics of E-cadherin and N-cadherin were developed by introducing a light responsive non-natural amino acid, His[Ru(bpy)2PPh3] at the His residue of the HAV cadherin binding motif. These peptides were immobilized on poly(acrylamide) hydrogels and cadherin-mediated cell adhesion and derived cellular responses as a function of the activity of the peptide were studied. Flat and micropatterned hydrogels were used to reconstruct polarized cellular microenvironments, as they occur in epithelial or musculoskeletal tissues. Cellular morphology on the novel cadherin mimetic hydrogels was explored. Light-regulated myogenic differentiation of C2C12 cells by temporal control of N-cadherin peptide presentation was demonstrated using the photoactivatable N-cadherin mimetic peptide.},
  language  = {en}
}