TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Flormann, Daniel A.
A1  - Anton, C.
A1  - Pohland, M.O.
A1  - Bautz, Y.
A1  - Kaub, Kevin
A1  - Terriac, Emmanuel
A1  - Schäffer, T.E.
A1  - Rheinlaender, J.
A1  - Janshoff, Andreas
A1  - Ott, A.
A1  - Lautenschläger, Franziska
T1  - Oscillatory Microrheology, Creep Compliance and Stress Relaxation of Biological Cells Reveal Strong Correlations as Probed by Atomic Force Microscopy
JF  - Frontiers in Physics
N2  - The mechanical properties of cells are important for many biological processes, including wound healing, cancers, and embryogenesis. Currently, our understanding of cell mechanical properties remains incomplete. Different techniques have been used to probe different aspects of the mechanical properties of cells, among them microplate rheology, optical tweezers, micropipette aspiration, and magnetic twisting cytometry. These techniques have given rise to different theoretical descriptions, reaching from simple Kelvin-Voigt or Maxwell models to fractional such as power law models, and their combinations. Atomic force microscopy (AFM) is a flexible technique that enables global and local probing of adherent cells. Here, using an AFM, we indented single retinal pigmented epithelium cells adhering to the bottom of a culture dish. The indentation was performed at two locations: above the nucleus, and towards the periphery of the cell. We applied creep compliance, stress relaxation, and oscillatory rheological tests to wild type and drug modified cells. Considering known fractional and semi-fractional descriptions, we found the extracted parameters to correlate. Moreover, the Young’s modulus as obtained from the initial indentation strongly correlated with all of the parameters from the applied power-law descriptions. Our study shows that the results from different rheological tests are directly comparable. This can be used in the future, for example, to reduce the number of measurements in planned experiments. Apparently, under these experimental conditions, the cells possess a limited number of degrees of freedom as their rheological properties change.
KW  - cell mechanics
KW  - atomic force microscopy
KW  - cell rheology
KW  - power-law
KW  - microrheology
KW  - creep compliance
KW  - stress relaxation
KW  - AFM
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-3417
SN  - 2296-424X
SS  - 2296-424X
U6  - https://doi.org/10.3389/fphy.2021.711860
DO  - https://doi.org/10.3389/fphy.2021.711860
VL  - 9
IS  - 472
SP  - 711860_1
EP  - 711860_12
ER  -