TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Kiefer, Ruth
A1  - Jurisic, Marijas
A1  - Dahlem, Charlotte
A1  - Koch, Marcus
A1  - Schmitt, Manfred J.
A1  - Kiemer, Alexandra K.
A1  - Schneider, Marc
A1  - Breinig, Frank
T1  - Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae
JF  - Biotechnology and Bioengineering
N2  - Abstract Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly (lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a co-culture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency. This article is protected by copyright. All rights reserved.
Y1  - 2020
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-3820
SN  - 0006-3592
SS  - 0006-3592
U6  - https://doi.org/10.1002/bit.27226
DO  - https://doi.org/10.1002/bit.27226
VL  - 117
IS  - 3
SP  - 776
EP  - 788
ER  -