TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Peuschel, Henrike
A1  - Ruckelshausen, Thomas
A1  - Cavelius, Christian
A1  - Kraegeloh, Annette
T1  - Quantification of internalized silica nanoparticles via STED microscopy
JF  - BioMed Research International
N2  - The development of safe engineered nanoparticles (NPs) requires a detailed understanding of their interaction mechanisms on a cellular level. Therefore, quantification of NP internalization is crucial to predict the potential impact of intracellular NP doses, providing essential information for risk assessment as well as for drug delivery applications. In this study, the internalization of 25 nm and 85 nm silica nanoparticles (SNPs) in alveolar type II cells (A549) was quantified by application of super-resolution STED (stimulated emission depletion) microscopy. Cells were exposed to equal particle number concentrations (9.2x10 10 particles mL −1 ) of each particle size and the sedimentation of particles during exposure was taken into account. Microscopy images revealed that particles of both sizes entered the cells after 5 h incubation in serum supplemented and serum-free medium. According to the in vitro sedimentation, diffusion, and dosimetry (ISDD) model 20–27% of the particles sedimented. In comparison, 10 2 -10 3 NPs per cell were detected intracellularly serum-containing medium. Furthermore, in the presence of serum, no cytotoxicity was induced by the SNPs. In serum-free medium, large agglomerates of both particle sizes covered the cells whereas only high concentrations (≥ 3.8 × 10 12 particles mL −1 ) of the smaller particles induced cytotoxicity.
Y1  - 2015
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-4180
SN  - 2314-6133
SS  - 2314-6133
U6  - https://doi.org/10.1155/2015/961208
DO  - https://doi.org/10.1155/2015/961208
VL  - 2015
SP  - Article ID 961208
ER  -