@article{RohlesGlaeserKohlstedtetal.2018,
  author    = {Christina Maria Rohles and Lars Gl{\"a}ser and Michael Kohlstedt and Gideon Gie{\"s}elmann and Samuel Pearson and Ar{\´a}nzazu del Campo B{\´e}cares and Judith Becker and Christoph Wittmann},
  title     = {A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicum},
  series   = {Green Chemistry},
  volume    = {20},
  number    = {20},
  publisher = {The Royal Society of Chemistry},
  issn      = {1463-9262},
  doi       = {10.1039/c8gc01901k},
  url       = {https://nbn-resolving.org/urn:nbn:de:bsz:291:415-4418},
  pages     = {4662 -- 4674},
  year      = {2018},
  abstract  = {In the present work, we established the bio-based production of glutarate, a carbon-5 dicarboxylic acid with recognized value for commercial plastics and other applications, using metabolically engineered Corynebacterium glutamicum. The mutant C. glutamicum AVA-2 served as a starting point for strain development, because it secreted small amounts of glutarate as a consequence of its engineered 5-aminovalerate pathway. Starting from AVA-2, we overexpressed 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase (gabD) under the control of the constitutive tuf promoter to convert 5-aminovalerate further to glutarate. The created strain GTA-1 formed glutarate as a major product, but still secreted 5-aminovalerate as well. This bottleneck was tackled at the level of 5-aminovalerate re-import. The advanced strain GTA-4 overexpressed the newly discovered 5-aminovalerate importer NCgl0464 and formed glutarate from glucose in a yield of 0.27 mol mol−1. In a fed-batch process, GTA-4 produced more than 90 g L−1 glutarate from glucose and molasses based sugars in a yield of up to 0.70 mol mol−1 and a maximum productivity of 1.8 g L−1 h−1, while 5-aminovalerate was no longer secreted. The bio-based glutaric acid was purified to >99.9\% purity. Interfacial polymerization and melt polymerization with hexamethylenediamine yielded bionylon-6,5, a polyamide with a unique structu},
  language  = {en}
}