TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Rohles, Christina Maria
A1  - Gläser, Lars
A1  - Kohlstedt, Michael
A1  - Gießelmann, Gideon
A1  - Pearson, Samuel
A1  - del Campo Bécares, Aránzazu
A1  - Becker, Judith
A1  - Wittmann, Christoph
T1  - A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicum
JF  - Green Chemistry
N2  - In the present work, we established the bio-based production of glutarate, a carbon-5 dicarboxylic acid with recognized value for commercial plastics and other applications, using metabolically engineered Corynebacterium glutamicum. The mutant C. glutamicum AVA-2 served as a starting point for strain development, because it secreted small amounts of glutarate as a consequence of its engineered 5-aminovalerate pathway. Starting from AVA-2, we overexpressed 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase (gabD) under the control of the constitutive tuf promoter to convert 5-aminovalerate further to glutarate. The created strain GTA-1 formed glutarate as a major product, but still secreted 5-aminovalerate as well. This bottleneck was tackled at the level of 5-aminovalerate re-import. The advanced strain GTA-4 overexpressed the newly discovered 5-aminovalerate importer NCgl0464 and formed glutarate from glucose in a yield of 0.27 mol mol−1. In a fed-batch process, GTA-4 produced more than 90 g L−1 glutarate from glucose and molasses based sugars in a yield of up to 0.70 mol mol−1 and a maximum productivity of 1.8 g L−1 h−1, while 5-aminovalerate was no longer secreted. The bio-based glutaric acid was purified to >99.9% purity. Interfacial polymerization and melt polymerization with hexamethylenediamine yielded bionylon-6,5, a polyamide with a unique structu
Y1  - 2018
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-4418
SN  - 1463-9262
SS  - 1463-9262
U6  - https://doi.org/10.1039/c8gc01901k
DO  - https://doi.org/10.1039/c8gc01901k
VL  - 20
IS  - 20
SP  - 4662
EP  - 4674
PB  - The Royal Society of Chemistry
ER  -