TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Wu, Wenming A1 - Guijt, Rosanne M. A1 - Silina, Yuliya E. A1 - Koch, Marcus A1 - Manz, Andreas T1 - Plant leaves as templates for soft lithography JF - RSC Advances N2 - We report a simple fast, practical and effective method for the replication of the complex venation patterns of natural leaves into PDMS with accuracy down to a lateral size of 500 nm. Optimising the amount of crosslinker enabled the replication and sealing of the microvascular structures to yield enclosed microfluidic networks. The use of plant leaves as templates for soft lithography was demonstrated across over ten species and included reticulate, arcuate, pinnate, parallel and palmate venation patterns. SEM imaging revealed replication of the plants microscopic and sub-microscopic topography into the PDMS structures, making this method especially attractive for mimicking biological structures for in vitro assays. Flow analysis revealed that the autonomous liquid transport velocity in 1 st -order microchannel was 1.5-2.2 times faster than that in the 2 nd -order microchannels across three leaf types, with the sorptivity rule surprisingly preserved during self-powered flow through leaf-inspired vascularity from Carpinus betulus. Y1 - 2016 UN - https://nbn-resolving.org/urn:nbn:de:bsz:291:415-4960 SN - 2046-2069 SS - 2046-2069 U6 - https://doi.org/10.1039/c5ra25890a DO - https://doi.org/10.1039/c5ra25890a VL - 6 IS - 27 SP - 22469 EP - 22475 ER -