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Oscillatory Microrheology, Creep Compliance and Stress Relaxation of Biological Cells Reveal Strong Correlations as Probed by Atomic Force Microscopy

  • The mechanical properties of cells are important for many biological processes, including wound healing, cancers, and embryogenesis. Currently, our understanding of cell mechanical properties remains incomplete. Different techniques have been used to probe different aspects of the mechanical properties of cells, among them microplate rheology, optical tweezers, micropipette aspiration, and magnetic twisting cytometry. These techniques have given rise to different theoretical descriptions, reaching from simple Kelvin-Voigt or Maxwell models to fractional such as power law models, and their combinations. Atomic force microscopy (AFM) is a flexible technique that enables global and local probing of adherent cells. Here, using an AFM, we indented single retinal pigmented epithelium cells adhering to the bottom of a culture dish. The indentation was performed at two locations: above the nucleus, and towards the periphery of the cell. We applied creep compliance, stress relaxation, and oscillatory rheological tests to wild type and drug modified cells. Considering known fractional and semi-fractional descriptions, we found the extracted parameters to correlate. Moreover, the Young’s modulus as obtained from the initial indentation strongly correlated with all of the parameters from the applied power-law descriptions. Our study shows that the results from different rheological tests are directly comparable. This can be used in the future, for example, to reduce the number of measurements in planned experiments. Apparently, under these experimental conditions, the cells possess a limited number of degrees of freedom as their rheological properties change.

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Document Type:Article
Author:Daniel A. FlormannORCiD, C. Anton, M.O. Pohland, Y. Bautz, Kevin Kaub, Emmanuel TerriacORCiD, T.E. Schäffer, J. Rheinlaender, Andreas JanshoffORCiD, A. Ott, Franziska LautenschlägerORCiD
Parent Title (English):Frontiers in Physics
First Page:711860_1
Last Page:711860_12
Year of first Publication:2021
Release Date:2022/11/18
atomic force microscopy; cell mechanics; cell rheology; creep compliance; microrheology; power-law; stress relaxation
Impact:03.718 (2021)
Funding Information:Deutsche Forschungsgemeinschaft (SPP1782 CRC/SFB 1027)
Open Access:Open Access
Signature:INM 2021/091
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International