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A high-throughput 3D kinetic killing assay

  • In vivo, immune killer cells must infiltrate into tissues and search for their cognate target cells in 3D environments. To investigate the cytotoxic function of immune killer cells, there is currently a significant need for an in vitro kinetic assay that resembles 3D in vivo features. Our work presents a high-throughput kinetic killing assay in 3D that is a robust and powerful tool for evaluating the killing efficiency of immune killer cells, as well as the viability of tumor cells under in vivo-like conditions. This assay holds particular value for assessing primary human CTLs and NK cells and can also be applied to primary murine killer cells. By utilizing collagen concentrations to mimic healthy tissue, soft tumors, and stiff tumors, this assay enables the evaluation of cell function and behavior in physiologically and pathologically relevant scenarios, particularly in the context of solid tumors. Furthermore, this assay shows promise as a personalized strategy for selecting more effective drugs/treatments against tumors, using primary immune cells for individual patients to achieve improved clinical outcomes.

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Metadaten
Document Type:Preprint
Author:Renping ZhaoORCiD, Archana K. YanamandraORCiD, Bin QuORCiD
URN:urn:nbn:de:bsz:291:415-6877
DOI:https://doi.org/10.1101/2023.04.11.536280
Parent Title (English):bioRxiv
First Page:1
Last Page:8
Language:English
Year of Completion:2023
Date of first Publication:2023/04/13
Release Date:2023/05/31
Tag:3D matrix; cytotoxicity assay; high content imaging; high throughput; kinetics
Funding Information:Deutsche Forschungsgemeinschaft (SFB 1027)
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
Signature:INM 2023/065_preprint
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International