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T cell stiffness is enhanced upon formation of immunological synapse

  • T cells are activated by cognate target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we established an atomic force microscopy (AFM)-based elasticity mapping method on whole T cells to obtain an overview of the stiffness with a resolution of ~ 60 nm. Using Jurkat T-cells and primary human CD4+ T cells, we show that in the T cells in contact with functionalized surfaces, the lamellipodia are stiffer than the cell body. Upon IS formation, T cell stiffness is substantially enhanced both at the lamellipodia and in cell body. Chelation of intracellular Ca2+ abolishes IS-induced stiffening at the lamellipodia but has no influence on cell body-stiffening, suggesting different regulatory mechanism of IS-induced stiffening between the lamellipodia and the cell body.Competing Interest StatementThe authors have declared no competing interest.

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Metadaten
Document Type:Preprint
Author:Philipp JungORCiD, Xiangda Zhou, Markus BischoffORCiD, Bin QuORCiD
URN:urn:nbn:de:bsz:291:415-7089
DOI:https://doi.org/10.1101/2021.01.06.425650
Parent Title (English):bioRxiv
First Page:1
Last Page:17
Language:English
Date of first Publication:2021/01/08
Release Date:2023/06/19
Tag:biophysics
Research Groups:Fellow
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
Signature:INM 2021/040
Licence (German):License LogoCreative Commons - CC BY-NC - Namensnennung - Nicht kommerziell 4.0 International