Volltext-Downloads (blau) und Frontdoor-Views (grau)

High Glucose Enhances Cytotoxic T Lymphocyte-Mediated Cytotoxicity

  • Cytotoxic T lymphocytes (CTLs) are key players to eliminate tumorigenic or pathogen-infected cells using lytic granules (LG) and Fas ligand (FasL) pathways. Depletion of glucose leads to severely impaired cytotoxic function of CTLs. However, the impact of excessive glucose on CTL functions still remains largely unknown. Here we used primary human CD8(+) T cells, which were stimulated by CD3/CD28 beads and cultured in medium either containing high glucose (HG, 25 mM) or normal glucose (NG, 5.6 mM). We found that in HG-CTLs, glucose uptake and glycolysis were enhanced, whereas proliferation remained unaltered. Furthermore, CTLs cultured in HG exhibited an enhanced CTL killing efficiency compared to their counterparts in NG. Unexpectedly, expression of cytotoxic proteins (perforin, granzyme A, granzyme B and FasL), LG release, cytokine/cytotoxic protein release and CTL migration remained unchanged in HG-cultured CTLs. Interestingly, additional extracellular Ca2+ diminished HG-enhanced CTL killing function. Our findings suggest that in an environment with excessive glucose, CTLs could eliminate target cells more efficiently, at least for a certain period of time, in a Ca2+-dependent manner.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar


Document Type:Article
Author:Jie Zhu, Wenjuan Yang, Xiangda Zhou, Dorina Zöphel, Leticia Soriano-BaguetORCiD, Denise Dolgener, Christopher CarleinORCiD, Chantal Hof, Renping ZhaoORCiD, Shandong Ye, Eva C. SchwarzORCiD, Dirk BrennerORCiD, Leticia Prates RomaORCiD, Bin QuORCiD
Parent Title (English):Frontieres in Immunology
First Page:10
Year of first Publication:2021
Release Date:2022/08/16
Tag:Cytotoxic T lymphocytes; Cytotoxicity; Glucose; Glycolysis; High clucose
Impact:08.786 (2021)
Funding Information:Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 1027 project A2 to BQ, TRR219 (M04) to LP); INM Fellow, and HOMFOR2019; Forschungsgroßgeräte (GZ: INST 256/423-1 FUGG and GZ: INST 256/419-1 FUGG); FNR, respectively by the PRIDE (PRIDE/11012546/NEXTIMMUNE) and the ATTRACT program (A14/BM/7632103
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
Signature:INM 2021/074
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International