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Role of actin cytoskeleton in cargo delivery mediated by vertically aligned silicon nanotubes

  • Nanofabrication technologies have been recently applied to the development of engineered nano–bio interfaces for manipulating complex cellular processes. In particular, vertically configurated nanostructures such as nanoneedles (NNs) have been adopted for a variety of biological applications such as mechanotransduction, biosensing, and intracellular delivery. Despite their success in delivering a diverse range of biomolecules into cells, the mechanisms for NN-mediated cargo transport remain to be elucidated. Recent studies have suggested that cytoskeletal elements are involved in generating a tight and functional cell–NN interface that can influence cargo delivery. In this study, by inhibiting actin dynamics using two drugs—cytochalasin D (Cyto D) and jasplakinolide (Jas), we demonstrate that the actin cytoskeleton plays an important role in mRNA delivery mediated by silicon nanotubes (SiNTs). Specifically, actin inhibition 12 h before SiNT-cellular interfacing (pre-interface treatment) significantly dampens mRNA delivery (with efficiencies dropping to 17.2% for Cyto D and 33.1% for Jas) into mouse fibroblast GPE86 cells, compared to that of untreated controls (86.9%). However, actin inhibition initiated 2 h after the establishment of GPE86 cell–SiNT interface (post-interface treatment), has negligible impact on mRNA transfection, maintaining > 80% efficiency for both Cyto D and Jas treatment groups. The results contribute to understanding potential mechanisms involved in NN-mediated intracellular delivery, providing insights into strategic design of cell–nano interfacing under temporal control for improved effectiveness.

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Document Type:Article
Author:Yaping ChenORCiD, Hao Zhe YohORCiD, Ali-Reza Shokouhi, Takahide Murayama, Koukou Suu, Yasuhiro Morikawa, Nicolas H. VoelckerORCiD, Roey ElnathanORCiD
Parent Title (English):Journal of Nanobiotechnology
First Page:406
Year of first Publication:2022
Release Date:2022/09/28
Tag:actin inhibition; cytoskeleton; intracellular delivery; nanoinjection; silicone nanotubes
Impact:09.429 (2021)
Funding Information:Australian Government (ARC DECRA project number: DE170100021)
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
Signature:INM 2022/084
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International