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Endothelial cell spreading on lipid bilayers with combined integrin and cadherin binding ligands

  • Endothelial cells play a central role in the vascular system, where their function is tightly regulated by both cell-extracellular matrix (e.g., via integrins) and cell–cell interactions (e.g., via cadherins). In this study, we incorporated cholesterol-modified integrin and N-cadherin peptide binding ligands in fluid supported lipid bilayers. Human umbilical vein endothelial cell adhesion, spreading and vinculin localization in these cells were dependent on ligand density. One composition led to observe a higher extent of cell spreading, where cells exhibited extensive lamellipodia formation and a qualitatively more distinct N-cadherin localization at the cell periphery, which is indicative of N-cadherin clustering and a mimic of cell–cell contact formation. The results can be used to reconstitute the endothelial-pericyte interface on biomedical devices and materials.

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Document Type:Article
Author:Gülistan KocerORCiD, Ines M. C. Albino, Mark L. Verheijden, Pascal JonkheijmORCiD
Parent Title (English):Bioorganic & Medicinal Chemistry
First Page:116850
Year of first Publication:2022
Release Date:2022/10/21
Tag:cell-instructive; cell-surface interactions; peptide ligands; supported lipid bilayers; surface modification
Impact:03.461 (2021)
Funding Information:Netherlands Organization for Scientific Research (NWO) (NWO-VIDI 723.012.106)
Groups:Dynamische Biomaterialien
DDC classes:600 Technik, Medizin, angewandte Wissenschaften / 610 Medizin, Gesundheit
Open Access:Open Access
Signature:INM 2022/108
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International