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Enhanced uptake and siRNA-mediated knockdown of a biologically relevant gene using cyclodextrin polyrotaxane

  • Ideal cationic polymers for siRNA delivery could result in its enhanced cellular internalization, escape from endosomal degradation, and rapid release in cell cytoplasm, to facilitate knockdown of the target gene. In this study, we have investigated the ability of an in-house synthesized cationic polyrotaxane to bind siRNA into nanometric complexes. This polymer, which had earlier shown improved transfection of model siRNA (luciferase), was used to improve the cellular internalization of the siRNA molecule with therapeutic implications. In cellular assays, the polymer enhanced the knockdown of a gene involved in the pathogenesis of tuberculosis, when the nanocomplexes were compared with free siRNA. The efficacy and cellular non-toxicity of this polymer encourage its further exploitation in animal models of tuberculosis and other intracellular bacterial infections.

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Document Type:Article
Author:Prajakta Dandekar, Ratnesh JainORCiD, Manuel Keil, Brigitta LoretzORCiD, Marcus Koch, Gerhard WenzORCiD, Claus-Michael LehrORCiD
Parent Title (English):Journal of Materials Chemistry B
First Page:2590
Last Page:2598
Year of first Publication:2015
Date of final exam:2015/02/05
Release Date:2022/11/18
Impact:04.872 (2015)
Funding Information:European Respiratory Society/Marie Curie Joint Research Fellowship – Number MC [1634]-2010. European Respiratory Society and the European Community's Seventh Framework Programme FP7/2007-2013-Marie Curie Actions under grant agreement RESPIRE, PCOFUND-GA-2008-229571. Alexander von Humboldt Foundation's postdoctoral fellowship.
Groups:Physikalische Analytik
Open Access:Open Access
Signature:INM 2015/30
Licence (German):License LogoCreative Commons - CC BY-NC - Namensnennung - Nicht kommerziell 4.0 International