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Analyses and localization of pectin-like carbohydrates in cell wall and mucilage of the green alga Netrium digitus

  • The unicellular, simply shaped desmid Netrium digitus inhabiting acid bog ponds grows in two phases. Prior to division, the cell elongates at its central zone, whereas in a second phase, polar tip growth occurs. Electron microscopy demonstrates that Netrium is surrounded by a morphologically homogeneous cell wall, which lacks pores. Immunocytochemical and biochemical analyses give insight into physical wall properties and, thus, into adaptation to the extreme environment. The monoclonal antibodies JIM5 and JIM7 directed against pectic epitopes with different degrees of esterification label preferentially growing wall zones in Netrium. In contrast, 2F4 marks the cell wall only after experimental de-esterification. Electron energy loss spectroscopy reveals Ca-binding capacities of pectins and gives indirect evidence for the degree of their esterification. An antibody raised against Netrium mucilage is not only specific to mucilage but also recognizes wall components in transmission electron microscopy and dot blots. These results indicate a smooth transition between mucilage and the cell wall in Netrium.

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Document Type:Article
Author:Magdalena Eder, Ursula Lütz-Meindl
Parent Title (English):Protoplasma
First Page:25
Last Page:38
Year of first Publication:2010
Release Date:2022/11/18
Tag:TEM; alga; cell wall; confocal laser scanning microscopy; mucilage; pectins
Impact:01.488 (2010)
Funding Information:University of Salzburg and the Austrian Science Fund project P15849 and P 18869-B16
Open Access:Open Access
Signature:INM 2010/53
Licence (German):License LogoCreative Commons - CC BY-NC - Namensnennung - Nicht kommerziell 4.0 International