Volltext-Downloads (blau) und Frontdoor-Views (grau)

Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis

  • Summary Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar


Document Type:Article
Author:Aleeza FarrukhORCiD, Felipe Ortega, Wenqiang Fan, Nicolás Marichal, Julieta I. PaezORCiD, Benedikt Berninger, Aránzazu del Campo BécaresORCiD, Marcelo J. Salierno
Parent Title (English):Stem Cell Reports
First Page:1432
Last Page:1440
Year of first Publication:2017
Release Date:2022/11/18
Tag:IKVAV; bioengineering; biomaterials; cell differentiation; hydrogels; laminin; neural stem cells; neurogenesis; polylysine; β-integrin
Impact:06.537 (2017)
Groups:Dynamische Biomaterialien
Open Access:Open Access
Signature:INM 2017/090
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International