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Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration

  • Summary In development, wound healing, and cancer metastasis, vertebrate cells move through 3D interstitial matrix, responding to chemical and physical guidance cues. Protrusion at the cell front has been extensively studied, but the retraction phase of the migration cycle is not well understood. Here, we show that fast-moving cells guided by matrix cues establish positive feedback control of rear retraction by sensing membrane tension. We reveal a mechanism of rear retraction in 3D matrix and durotaxis controlled by caveolae, which form in response to low membrane tension at the cell rear. Caveolae activate RhoA-ROCK1/PKN2 signaling via the RhoA guanidine nucleotide exchange factor (GEF) Ect2 to control local F-actin organization and contractility in this subcellular region and promote translocation of the cell rear. A positive feedback loop between cytoskeletal signaling and membrane tension leads to rapid retraction to complete the migration cycle in fast-moving cells, providing directional memory to drive persistent cell migration in complex matrices.

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Metadaten
Document Type:Article
Author:Joseph H.R. Hetmanski, Henry de Belly, Ignacio Busnelli, Thomas Waring, Roshna Vakkeel NairORCiD, Vanesa Sokleva, Oana DobreORCiD, Angus Cameron, Nils Gauthier, Christophe Lamaze, Aránzazu del Campo BécaresORCiD, Tobias Starborg, Tobias Zech, Jacky G. Goetz, Ewa K. Paluch, Jean-Marc Schwartz, Patrick T. Caswell
URN:urn:nbn:de:bsz:291:415-3610
URL:http://www.sciencedirect.com/science/article/pii/S153458071930735X
DOI:https://doi.org/10.1016/j.devcel.2019.09.006
ISSN:1534-5807
Parent Title (English):Developmental Cell
Volume:51
Issue:4
First Page:460
Last Page:475
Language:English
Year of first Publication:2019
Release Date:2022/11/18
Tag:RhoGTPase; caveolae; cell invasion; cell migration; cytoskeleton; durotaxis; extracellular matrix; membrane tension
Impact:10.092 (2019)
Groups:Dynamische Biomaterialien
Researchfields:Biogrenzflächen
Open Access:Open Access
Signature:INM 2019/160
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International