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A rational design of a cancer-specific and lysosome-targeted fluorescence nanoprobe for glutathione imaging in living cells

  • Developing a versatile probe for targeting the lysosomes of specific cancer cells and subsequently detecting glutathione (GSH) levels is critical in disclosing the roles of GSH in the lysosomal oxidative stress of cancer cells. Herein, we demonstrate an efficient strategy for the preparation of a dual-targeting (both cancer cell- and lysosome-targeting) fluorescence nanoprobe (DTFN) that enables the imaging of GSH in the lysosomes of specific cancer cells. The nanoprobe (DTFN) is obtained by combining folic acid (FA)-modified photostable aggregation-induced emission dots with GSH-responsive manganese dioxide (MnO2) nanosheets via electrostatic interactions. DTFN has outstanding characteristics of good water dispersity, delightful photostability, shorter responsive time ( ∼ 5 min) and wide pH-response range. Intracellular experiments showed that the as-prepared DTFN could be preferentially internalized into a folate receptor (FR)-positive cancer cells via the FR-mediated endocytosis. Subsequently, with the aid of the positively charged amino moiety of the nanoprobe, DTFN can selectively accumulate in lysosomes and successfully achieve the real-time imaging of the lysosomal GSH levels in FR-positive cancer cells. This study highlights a strategy to design a versatile dual-targeting fluorescence probe for enhanced cancer imaging.

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Document Type:Article
Author:Yongxiang Hong, Hong WangORCiD, Peisheng ZhangORCiD, Chonghua Zhang, Shu Chen, Rongjin Zeng, Jiaxi CuiORCiD, Jian ChenORCiD
Parent Title (English):Materials Advances
First Page:1739
Last Page:1744
Year of first Publication:2020
Release Date:2022/11/18
Funding Information:National Natural Science Foundation of China (21705040517730565160306751873058) Natural Science Foundation of Hunan Province (2020JJ30212018JJ3143) China Postdoctoral Science Foundation (2018T110824) Education Department of Hunan Province (19B204) State Key Laboratory of Chemo/Biosensing and Chemometrics (2018011) State Key Laboratory of Luminescent Materials and Devices (2019-skllmd-09)
Open Access:Open Access
Signature:INM 2020/121
Licence (German):License LogoCreative Commons - CC BY-NC - Namensnennung - Nicht kommerziell 4.0 International