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Targeted delivery of functionalized PLGA nanoparticles to macrophages by complexation with the yeast Saccharomyces cerevisiae

  • Abstract Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly (lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a co-culture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency. This article is protected by copyright. All rights reserved.

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Document Type:Article
Author:Ruth KieferORCiD, Marijas Jurisic, Charlotte DahlemORCiD, Marcus Koch, Manfred J. Schmitt, Alexandra K. KiemerORCiD, Marc SchneiderORCiD, Frank BreinigORCiD
Parent Title (English):Biotechnology and Bioengineering
First Page:776
Last Page:788
Year of first Publication:2020
Release Date:2022/11/18
Impact:04.530 (2020)
Funding Information:Deutsche Forschungsgemeinschaft (KI702); Saarland Landesforschungsförderung (LFFP 1303, LFFP 17/08)
Scientific Units:Physical Analytics
Open Access:Open Access
Signature:INM 2020/010
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International