Volltext-Downloads (blau) und Frontdoor-Views (grau)

Zinc finger of arabidopsis thaliana12 (ZAT12) interacts with fer-like iron deficiency-induced transcription facotr (FIT) linking iron deficiency and oxidative stress responses

  • Plants grown under iron (Fe)-deficient conditions induce a set of genes that enhance the efficiency of Fe uptake by the roots. In Arabidopsis (Arabidopsis thaliana), the central regulator of this response is the basic helix-loop-helix transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT). FIT activity is regulated by protein-protein interactions, which also serve to integrate external signals that stimulate and possibly inhibit Fe uptake. In the search of signaling components regulating FIT function, we identified ZINC FINGER OF ARABIDOPSIS THALIANA12 (ZAT12), an abiotic stress-induced transcription factor. ZAT12 interacted with FIT, dependent on the presence of the ethylene-responsive element-binding factor-associated amphiphilic repression motif. ZAT12 protein was found expressed in the root early differentiation zone, where its abundance was modulated in a root layer-specific manner. In the absence of ZAT12, FIT expression was upregulated, suggesting a negative effect of ZAT12 on Fe uptake. Consistently, zat12 loss-of-function mutants had higher Fe content than the wild type at sufficient Fe. We found that under Fe deficiency, hydrogen peroxide (H 2 O 2 ) levels were enhanced in a FIT-dependent manner. FIT protein, in turn, was stabilized by H 2 O 2 but only in the presence of ZAT12, showing that H 2 O 2 serves as a signal for Fe deficiency responses. We propose that oxidative stress-induced ZAT12 functions as a negative regulator of Fe acquisition. A model where H 2 O 2 mediates the negative regulation of plant responses to prolonged stress might be applicable to a variety of stress conditions.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar


Document Type:Article
Author:Cham Thi Tuyet Le, Tzvetina BrumbarovaORCiD, Rumen IvanovORCiD, Claudia Stoof, Eva Weber, Julia Mohrbacher, Claudia Fink-Straube, Petra BauerORCiD
Parent Title (English):Plant Physiology
First Page:540
Last Page:557
Year of first Publication:2016
Release Date:2022/11/18
Impact:06.456 (2016)
Groups:Chemische Analytik
Open Access:Open Access
Signature:INM 2016/11
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International