Recombinant phage coated 1D Al 2 O 3 nanostructures for controlling the adhesion and proliferation of endothelial cells
- A novel synthesis of a nanostructured cell adhesive surface is investigated for future stent developments. One-dimensional (1D) Al 2 O 3 nanostructures were prepared by chemical vapor deposition of a single source precursor. Afterwards, recombinant filamentous bacteriophages which display a short binding motif with a cell adhesive peptide (RGD) on p3 and p8 proteins were immobilized on these 1D Al 2 O 3 nanostructures by a simple dip-coating process to study the cellular response of human endothelial EA hy.926. While the cell density decreased on as-deposited 1D Al 2 O 3 nanostructures, we observed enhanced cell proliferation and cell-cell interaction on recombinant phage overcoated 1D Al 2 O 3 nanostructures. The recombinant phage overcoating also supports an isotropic cell spreading rather than elongated cell morphology as we observed on as-deposited Al 2 O 3 1D nanostructures.
| Document Type: | Article |
|---|---|
| Author: | Juseok Lee, Hojeong Jeon, Ayman HaidarORCiD, Hashim Abdul-Khaliq, Michael Veith, Oral C. AktasORCiD, Youngjun Kim |
| URN: | urn:nbn:de:bsz:291:415-3924 |
| DOI: | https://doi.org/10.1155/2015/909807 |
| ISSN: | 2314-6133 |
| Parent Title (English): | BioMed Research International |
| Volume: | 2015 |
| First Page: | 909807 |
| Language: | English |
| Year of first Publication: | 2015 |
| Release Date: | 2022/11/18 |
| Impact: | 02.134 (2015) |
| Funding Information: | Korea-EU Joint Research Program 2014 of the National Research Council of Science and Technology (NST)) between Leibniz Institute for New Materials and Korea Institute of Science and Technology Europe |
| Open Access: | Open Access |
| Signature: | INM 2015/048 |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
