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Toward Light-Regulated Living Biomaterials

  • Abstract Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)-based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin-specific miniprotein on the outer membrane of an endotoxin-free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light-activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner.

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Metadaten
Document Type:Article
Author:Shrikrishnan SankaranORCiD, Shifang Zhao, Christina Muth, Julieta I. PaezORCiD, Aránzazu del Campo BécaresORCiD
URN:urn:nbn:de:bsz:291:415-4469
DOI:https://doi.org/10.1002/advs.201800383
Parent Title (English):Advanced Science
Volume:5
Issue:8
Pagenumber:1800383
Language:English
Year of first Publication:2018
Release Date:2022/11/18
Impact:12.441 (2018)
Scientific Units:Dynamical Biomaterials
Open Access:Open Access
Signature:INM 2018/078
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International