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Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

  • Little is known about the simultaneous uptake of different engineered nanoparticle types, as it can be expected in our daily life. In order to test such co-exposure effects, murine macrophages (J774A.1 cell line) were incubated with gold (AuNPs) and iron oxide nanoparticles (FeOxNPs) either alone or combined. Environmental scanning electron microscopy revealed that single NPs of both types bound within minutes on the cell surface but with a distinctive difference between FeOxNPs and AuNPs. Uptake analysis studies based on laser scanning microscopy, transmission electron microscopy, and inductively coupled plasma optical emission spectrometry revealed intracellular appearance of both NP types in all exposure scenarios and a time-dependent increase. This increase was higher for both AuNPs and FeOxNPs during co-exposure. Cells treated with endocytotic inhibitors recovered after co-exposure, which additionally hinted that two uptake mechanisms are involved. Cross-talk between uptake pathways is relevant for toxicological studies: Co-exposure acts as an uptake accelerant. If the goal is to maximize the cellular uptake, e.g., for the delivery of pharmaceutical agents, this can be beneficial. However, co-exposure should also be taken into account in the case of risk assessment of occupational settings. The demonstration of co-exposure-invoked pathway interactions reveals that synergetic nanoparticle effects, either positive or negative, must be considered for nanotechnology and nanomedicine in particular to develop to its full potential.

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Document Type:Article
Author:Dimitri Vanhecke, Dagmar A. Kuhn, Dorleta Jimenez de Aberasturi, Sandor Balog, Ana Milosevic, Dominic Urban, Diana B. PeckysORCiD, Niels de JongeORCiD, Wolfgang J. Parak, Alke Petri-Fink, Barbara Rothen-Rutishauser
Parent Title (English):Beilstein Journal of Nanotechnology
First Page:2396
Last Page:2409
Year of first Publication:2017
Release Date:2022/11/18
Tag:Endocytosis; co-exposure; live cell imaging; nanoparticles; quantitative microscopy
Impact:02.968 (2017)
Funding Information:Deutsche Forschungsgemeinschaft (DFG, SPP 1313 to B.R.R. and PA 794/25-1 to W.J.P.), the Leibniz Competition 2014, the European Commission (grant FutureNanoNeeds to W.J.P.), the Adolphe Merkle Foundation, the Swiss National Science Foundation (Grant # 310030_159847/1) and by the Swiss National Science Foundation through the National Centre of Competence in Research “Bio-Inspired Materials”.
Groups:Innovative Elektronenmikroskopie
Open Access:Open Access
Signature:INM 2017/075
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International