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Thiol-Methylsulfone Based Hydrogels: Enhanced Control on Gelation Kinetics for 3D Cell Encapsulation

  • Hydrogels are useful temporal matrices for cell culture technologies. The successful mixing and encapsulation of cells within the gel requires the selection of efficient and cytocompatible gelation reactions occurring in the minute timescale under physiological conditions. The thiol-methylsulfonyl (MS) chemical reaction is introduced here as a novel chemistry to encapsulate cells in polymeric matrices. Thiol-MS crosslinking does not require a light activation step and can occur within the seconds-to-minutes timescale by adjusting the pH in the physiological range 8.0-6.6. This reaction is cytocompatible and the reaction product is hydrolytically stable in cell culture media up to 4 weeks. Cell encapsulation protocols enabling comfortable handling and yielding homogenous distribution of the embedded cells are described. All these features are relevant for the application of this crosslinking reaction to biomedical scenarios. Finally, this manuscript also compares the performance of thiol-MS hydrogels with the established thiol-maleimide and thiol-vinylsulfone hydrogels. The benefit of thiol-MS crosslinking in terms of control over hydrogelation kinetics is demonstrated.

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Metadaten
Document Type:Preprint
Author:Julieta I. PaezORCiD, Aleeza FarrukhORCiD, Małgorzata K. Włodarczyk-BiegunORCiD, Aránzazu del Campo BécaresORCiD
URN:urn:nbn:de:bsz:291:415-5326
DOI:https://doi.org/10.26434/chemrxiv.8971136.v1
Parent Title (English):ChemRxiv
First Page:1
Last Page:20
Language:English
Date of Publication (online):2019/07/22
Year of first Publication:2019
Release Date:2022/12/21
Tag:3D cell culture; aromatic methylsulfones; coupling under physiological conditions; gelation kinetics; thiol-mediated chemistry
Funding Information:European Union’s Horizon 2020 research and innovation programme under the FET PROACTIVE grant agreement no. 731957 (Mechano-Control); DFG (Project no. 422041745)
Scientific Units:Dynamical Biomaterials
DDC classes:500 Naturwissenschaften und Mathematik / 540 Chemie
Open Access:Open Access
Signature:INM 2019/108_preprint
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International