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Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity

  • Engineered living materials (ELMs) are a new class of materials in which living organism incorporated into diffusive matrices uptake a fundamental role in material's composition and function. Understanding how the spatial confinement in 3D can regulate the behavior of the embedded cells is crucial to design and predict ELM's function, minimize their environmental impact and facilitate their translation into applied materials. This study investigates the growth and metabolic activity of bacteria within an associative hydrogel network (Pluronic-based) with mechanical properties that can be tuned by introducing a variable degree of acrylate crosslinks. Individual bacteria distributed in the hydrogel matrix at low density form functional colonies whose size is controlled by the extent of permanent crosslinks. With increasing stiffness and elastic response to deformation of the matrix, a decrease in colony volumes and an increase in their sphericity are observed. Protein production follows a different pattern with higher production yields occurring in networks with intermediate permanent crosslinking degrees. These results demonstrate that matrix design can be used to control and regulate the composition and function of ELMs containing microorganisms. Interestingly, design parameters for matrices to regulate bacteria behavior show similarities to those elucidated for 3D culture of mammalian cells.

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Metadaten
Document Type:Article
Author:Shardul BhusariORCiD, Shrikrishnan SankaranORCiD, Aránzazu del Campo BécaresORCiD
URN:urn:nbn:de:bsz:291:415-632
DOI:https://doi.org/10.1002/advs.202106026
ISSN:2198-3844
Parent Title (English):Advanced Science
Volume:9
Issue:17
First Page:2106026
Language:English
Year of first Publication:2022
Release Date:2022/06/29
Tag:bacterial hydrogels; bacterial-materials interactions; cell encapsulation; dynamic hydrogel; engineered living material
Impact:17.521 (2021)
Funding Information:Leibniz-Gemeinschaft. Grant Number: Leibniz Science Campus on Living Therapeutic Materials (LifeMat) Deutsche Forschungsgemeinschaft. Grant Number: SFB1027
Scientific Units:Dynamical Biomaterials
Bioprogrammable Materials
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
Signature:INM 2022/047
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International