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Light-Sheet Scattering Microscopy to Visualize Long-Term Interactions Between Cells and Extracellular Matrix

  • Visualizing interactions between cells and the extracellular matrix (ECM) mesh is important to understand cell behavior and regulatory mechanisms by the extracellular environment. However, long term visualization of three-dimensional (3D) matrix structures remains challenging mainly due to photobleaching or blind spots perpendicular to the imaging plane. Here, we combine label-free light-sheet scattering microcopy (LSSM) and fluorescence microscopy to solve these problems. We verified that LSSM can reliably visualize structures of collagen matrices from different origin including bovine, human and rat tail. The quality and intensity of collagen structure images acquired by LSSM did not decline with time. LSSM offers abundant wavelength choice to visualize matrix structures, maximizing combination possibilities with fluorescently-labelled cells, allowing visualizing of long-term ECM-cell interactions in 3D. Interestingly, we observed ultrathin thread-like structures between cells and matrix using LSSM, which were not observed by normal fluorescence microscopy. Transient local alignment of matrix by cell-applied forces can be observed. In summary, LSSM provides a powerful and robust approach to investigate the complex interplay between cells and ECM.

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Document Type:Article
Author:Xiangda Zhou, Renping ZhaoORCiD, Archana K. YanamandraORCiD, Markus HothORCiD, Bin QuORCiD
Parent Title (English):Frontiers in immunology
First Page:828634
Year of first Publication:2022
Release Date:2022/05/06
Tag:ECM-cell interaction; label-free; light sheet scattering microsopy; long-term
GND Keyword:Immunologie
Impact:08.786 (2021)
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
This project was funded by the Deutsche Forschungsgemeinschaft (SFB 1027 Project A2 to BQ, A11 to MH, and GZ: INST 256/419-1 FUGG to MH), and by Bundesministerium für Bildung und Forschung (031L0133 to MH)
Signature:INM 2022/027
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International