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Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork

  • The permeability of the Human Trabecular Meshwork (HTM) regulates eye pressure via a porosity gradient across its thickness modulated by stacked layers of matrix fibrils and cells. Changes in HTM porosity are associated with increases in intraocular pressure and the progress of diseases like glaucoma. Engineered HTMs could help to understand the structure-function relation in natural tissues, and lead to new regenerative solutions. Here, melt electrowriting (MEW) is explored as a biofabrication technique to produce fibrillar, porous scaffolds that mimic the multilayer, gradient structure of native HTM. Poly(caprolactone) constructs with a height of 125-500 μm and fiber diameters of 10-12 μm are printed. Scaffolds with a tensile modulus between 5.6 and 13 MPa, and a static compression modulus in the range of 6-360 kPa are obtained by varying the scaffolds design, i.e., density and orientation of the fibers and number of stacked layers. Primary HTM cells attach to the scaffolds, proliferate, and form a confluent layer within 8-14 days, depending on the scaffold design. High cell viability and cell morphology close to that in the native tissue are observed. The present work demonstrates the utility of MEW to reconstruct complex morphological features of natural tissues.Competing Interest StatementThe authors have declared no competing interest.

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Document Type:Preprint
Author:Małgorzata K. Włodarczyk-BiegunORCiD, Maria VilliouORCiD, Marcus Koch, Christina Muth, Peixi Wang, Jenna Ott, Aránzazu del Campo BécaresORCiD
Parent Title (German):bioRxiv
Date of first Publication:2022/05/29
Release Date:2023/02/23
Funding Information:EU within BioSmartTrainee, the Marie Skłodowska-Curie Innovative Training School, No. 642861
Groups:Dynamische Biomaterialien
Physikalische Analytik
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Open Access:Open Access
Signature:INM 2022/005_preprint
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International