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Possibilities and limitations of photoactivatable cytochalasin D for the spatiotemporal regulation of actin dynamics

  • The study of the actin cytoskeleton and related cellular processes requires tools to specifically interfere withactin dynamics in living cell cultures, ideally with spatiotemporal control and compatible with real time imaging.A phototriggerable derivative of the actin disruptor Cytochalasin D (CytoD) is described and tested here. Itincludes a nitroveratryloxycarbonyl (Nvoc) photoremovable protecting group (PPG) at the hydroxyl group atC7 of CytoD. The attachment of the PPG renders Nvoc-CytoD temporarily inactive, and enables light-doseddelivery of the active drug CytoD to living cells. This article presents the full structural and physicochemicalcharacterization, the toxicity analysis. It is complemented with biological tests to show the time scales(seconds) and spatial resolution (cellular level) achievable with a UV source in a regular microscopy setup.

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Document Type:Preprint
Author:Roshna Vakkeel NairORCiD, Shifang Zhao, Emmanuel TerriacORCiD, Franziska LautenschlägerORCiD, Joseph H.R. Hetmanski, Patrick T. Caswell, Aránzazu del Campo BécaresORCiD
Parent Title (English):chemRxiv
Year of Completion:2020
Date of first Publication:2020/07/06
Release Date:2023/06/20
Tag:actin dynamics; cytochalasin D; photoregulated actin
Groups:Dynamische Biomaterialien
DDC classes:500 Naturwissenschaften und Mathematik / 540 Chemie
Open Access:Open Access
Signature:INM 2020/076_preprint
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International