Possibilities and limitations of photoactivatable cytochalasin D for the spatiotemporal regulation of actin dynamics
- The study of the actin cytoskeleton and related cellular processes requires tools to specifically interfere withactin dynamics in living cell cultures, ideally with spatiotemporal control and compatible with real time imaging.A phototriggerable derivative of the actin disruptor Cytochalasin D (CytoD) is described and tested here. Itincludes a nitroveratryloxycarbonyl (Nvoc) photoremovable protecting group (PPG) at the hydroxyl group atC7 of CytoD. The attachment of the PPG renders Nvoc-CytoD temporarily inactive, and enables light-doseddelivery of the active drug CytoD to living cells. This article presents the full structural and physicochemicalcharacterization, the toxicity analysis. It is complemented with biological tests to show the time scales(seconds) and spatial resolution (cellular level) achievable with a UV source in a regular microscopy setup.
Download full text files
Metrics
Statistics
| Document Type: | Preprint |
|---|---|
| Author: | Roshna Vakkeel NairORCiD, Shifang Zhao, Emmanuel TerriacORCiD, Franziska LautenschlägerORCiD, Joseph H.R. Hetmanski, Patrick T. Caswell, Aránzazu del Campo BécaresORCiD |
| URN: | urn:nbn:de:bsz:291:415-7063 |
| DOI: | https://doi.org/10.26434/chemrxiv.12609545.v1 |
| Parent Title (English): | chemRxiv |
| Issue: | V1 |
| Language: | English |
| Year of Completion: | 2020 |
| Date of first Publication: | 2020/07/06 |
| Release Date: | 2023/06/20 |
| Tag: | actin dynamics; cytochalasin D; photoregulated actin |
| Scientific Units: | Dynamic Biomaterials |
| DDC classes: | 500 Naturwissenschaften und Mathematik / 540 Chemie |
| Open Access: | Open Access |
| Signature: | INM 2020/076_preprint |
| Licence (German): |  Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
